I took a photo with my lens cap on, manual mode 1/4s, ISO100 and verified the hot pixel's there. With the lens still on, I went to manual (not auto) sensor cleaning in the XTi menu, started the procedure, I could hear the mirror flip up, I counted 5 seconds and turned the XTi off. Turned the XTi back on and with the lens cap still on, took another manual 1/4s, ISO100 shot and another at ISO1600. The red hot pixel is gone!