If you want to be safe, BACK UP whatever is on the drive you want to partition BEFORE attempting to partition it.
There used to be two very useful utility apps for OS X:
- iDefrag
- iPartition
Both were published by a company called Coriolis Systems that has now discontinued them, because changes in the Mac OS and the move from platter-based drives to SSDs has diminished the need for the above apps.
However, some folks may still have a need for them.
Coriolis has graciously made available all previous versions of their software.
You can freely download them from here:
iPartition can probably do what you want.
But again, to be safe, you should back up the drive first!