I just tried installing 10.12.x and also 10.13.6 on a samsung qvo 1tb drive in the sata 2 bay and both OS showed the "no entry" symbol on restarts , but not on cold boot. I have used this drive before in a 2013 imac with no problem. (obviously samsung evo and pro models work in a 5,1 no problem) I also found a similar post on reddit made by another mac pro5,1 owner about a week ago. I am guessing its a sata 3 to sata 2 issue., thus it does not occur on the newer imac sata bus.