Had a chance to add the Sapphire PULSE RX580 8GB back to the system this AM, with a modified install in slot 1. Troubleshooting the install issues from a card in slot 2 drooping into the GPU and an audio interference issue from another thread:
https://forums.macrumors.com/threads/rx580-audio-interference.2134677/
Put one thermal pad sheet (20x20x1mm) on top of each of the four bottom backplate screws, but was running into issues with the fit/install when used in combination with four stacked thermal pads on four different parts of the top of the GPU to prevent interference with the card in slot 2. Was creating a bridge to keep the end of the card in slot 2 out of the first GPU fan (closest to the ports).
Here's the original plan:
Was hearing some GPU fan interference. Immediately turned the machine off. Removed the GPU and the pads on the two screws closest to the PCIe connectors were already heavily torn apart. There's just not enough clearance on the PCIe connector side. Removed the ripped pads and removed two backplate screws on the GPU closest to the PCIe connectors. Left the thermal pads on top of the two other remaining backplate screws.
Forgot to take a photo of the changed top installation setup, but ended up ONLY using the top stacked thermal pads in-between the two GPU fans. Removed the two stacks on the side near the GPU ports. The card in slot 2 was not interfering in that area, only towards the middle/end of the first GPU fan (closest to the port side).
After reinstalling into the system, everything works fine. There are no GPU fan issues resulting from the card in slot 2.
The stacked thermal pads on the top of the GPU are four square 20x20x1mm stacked for approximately 4mm of height near the PCIe connector (the larger area of the GPU case). The spot near the middle of the two fans is one 20x20x1mm cut into 4 strips and stacked for approximately 4mm of height (one square cut in half, then each half cut in half again).
There is absolutely no audio interference. Using the same exact power cables and setup that I was using previously, except for the GPU swap. (This same exact setup was working 100% fine with the NVIDIA GTX 1080 FE installed.)
Hopefully will have some time to further test the RX580 throughout the week.