The only late 2009 iMac that is supported by the VBIOSes here is the 27" Core-i model, the iMac11,1 ; all other late 2009 models are iMac10,x .Some help please cause I'm running out of ideas, I must have installed a dozen of these cards by now and everytime it was a breeze, I always go for the K2100M and never had problems, I bought two this time (one for stock) flashed them both with C341A and installed them on an iMac 21.5 late 2009. I get 4 leds, chime and then the fans go full speed and the screen remains black, both new cards do the same. It doesn't seem to boot at all as when i press the power button it immediately powers off.
Edit: one thing I thought of right now is that the macOS is at mountain lion, I know that when the system is updated to High Sierra it gets an EFI update, is this needed for these cards to work?
The internal screen almost certainly won't work.
Anything but the latest EFI bootrom versions is unknown territory so the latest version is a must.
EDIT: As always you find all that information in the first post on this thread - so please excuse me for repeating that information here.